BD LSR Fortessa (16 colour Analyser) 4 lasers, 16 filters (see table below). Sample loading via tubes. Table of lasers and filters available: Laser. Filters. Example of Fluorochrome. Blue 488nm. B 710/50.
De analyserades med en FACS Fortessa flödescytometer (BD Biosciences, San micro-channel plate (MCP) detector and high magnification camera system.
Confirm the computer was OFF before you do this. Otherwise, switch OFF the computer first. Be aware that the lasers take about 15 minutes to warm up and stabilize. LSR Fortessa .
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Complete Optical Configuration of the Fortessa1 Our second Fortessa has exactly the same configuration as our first fortessa on the violet, yellow and red laser paths, but a different configuration on the blue laser and the addition of 2 channels on Ultra-Violet 355nm Optical Configuration of the Fortessa2 (our only UV laser instrument)
8 peak beads show fluorescence in all channels and allow to distinguish up to 8 separate populations depending on the channel resolution. 2 BD LSRFortessa Cell Analyzer User’s Guide What this guide covers This guide describes the procedures necessary to operate and maintain your BD LSRFortessa™ cell analyzer. Because many cytometer functions are controlled by BD FACSDiva™ software, this guide also contains information about software features required for basic cytometer setup and The LSR Fortessa uses BD FacsFlow as the sheath fluid. 20 L boxes of which are available in lab 1.47a (CIMR) and on the bench behind the machine (e6) Please contact Flow Core Unit staff if none are present The sheath fluid sits on the left of the FACS Flow Supply cart underneath the bench.
Aug 7, 2019 Page 2. Rev. 7 Aug 2019. Analyzers. LSR Fortessa. Analyzer. BD Biosciences. Laser Ex (nm) Em (nm). Channel. Fluorochromes. Ultra-violet.
BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b. LSR Fortessa . The LSR Fortessa offers 5 lasers and is able to detect up to 18 parameters (fluorochromes) at the same time!
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The LSR Fortessa is is equipped with four laser lines (488nm, 405nm, 561nm, and 640nm) and is capable of detecting 18 parameters; 2 scatter and 16 fluorescent detectors.
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Technical Bulletin. Standardizing Application Setup Across Multiple Flow Cytometers Using BD FACSDiva™ Version 6 Linearity maximum channel (the maximum acceptable signal level for good linearity)1 BD LSR Fortessa. PE -A. -557.
Analyzer. BD Biosciences.
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of the supernatant with a vacuum pump containing an eight-channel adaptor. FACS buffer and acquired on a LSR II Fortessa cell analyzer (BD Bioscience).
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Flow cytometry studies were performed using a LSRII Fortessa (Becton streptavidin) were obtained from BD Biosciences, eBioscience, or Biolegend 30 .
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